# NDB ID: PD0080

## Features

DNA single strand, modified

## Compound name

DNA POLYMERASE I (E.C.2.7.7.7)/DNA COMPLEX

## Sequence in asymmetric unit

- Chain A: T T U T
- Chain B: MET ILE SER TYR ASP ASN TYR VAL THR ILE LEU ASP GLU GLU THR LEU LYS ALA TRP ILE ALA LYS LEU GLU LYS ALA PRO VAL PHE ALA PHE ASP THR GLU THR ASP SER LEU ASP ASN ILE SER ALA ASN LEU VAL GLY LEU SER PHE ALA ILE GLU PRO GLY VAL ALA ALA TYR ILE PRO VAL ALA HIS ASP TYR LEU ASP ALA PRO ASP GLN ILE SER ARG GLU ARG ALA LEU GLU LEU LEU LYS PRO LEU LEU GLU ASP GLU LYS ALA LEU LYS VAL GLY GLN ASN LEU LYS TYR ASP ARG GLY ILE LEU ALA ASN TYR GLY ILE GLU LEU ARG GLY ILE ALA PHE ASP THR MET LEU GLU SER TYR ILE LEU ASN SER VAL ALA GLY ARG HIS ASP MET ASP SER LEU ALA GLU ARG TRP LEU LYS HIS LYS THR ILE THR PHE GLU GLU ILE ALA GLY LYS GLY LYS ASN GLN LEU THR PHE ASN GLN ILE ALA LEU GLU GLU ALA GLY ARG TYR ALA ALA GLU ASP ALA ASP VAL THR LEU GLN LEU HIS LEU LYS MET TRP PRO ASP LEU GLN LYS HIS LYS GLY PRO LEU ASN VAL PHE GLU ASN ILE GLU MET PRO LEU VAL PRO VAL LEU SER ARG ILE GLU ARG ASN GLY VAL LYS ILE ASP PRO LYS VAL LEU HIS ASN HIS SER GLU GLU LEU THR LEU ARG LEU ALA GLU LEU GLU LYS LYS ALA HIS GLU ILE ALA GLY GLU GLU PHE ASN LEU SER SER THR LYS GLN LEU GLN THR ILE LEU PHE GLU LYS GLN GLY ILE LYS PRO LEU LYS LYS THR PRO GLY GLY ALA PRO SER THR SER GLU GLU VAL LEU GLU GLU LEU ALA LEU ASP TYR PRO LEU PRO LYS VAL ILE LEU GLU TYR ARG GLY LEU ALA LYS LEU LYS SER THR TYR THR ASP LYS LEU PRO LEU MET ILE ASN PRO LYS THR GLY ARG VAL HIS THR SER TYR HIS GLN ALA VAL THR ALA THR GLY ARG LEU SER SER THR ASP PRO ASN LEU GLN ASN ILE PRO VAL ARG ASN GLU GLU GLY ARG ARG ILE ARG GLN ALA PHE ILE ALA PRO GLU ASP TYR VAL ILE VAL SER ALA ASP TYR SER GLN ILE GLU LEU ARG ILE MET ALA HIS LEU SER ARG ASP LYS GLY LEU LEU THR ALA PHE ALA GLU GLY LYS ASP ILE HIS ARG ALA THR ALA ALA GLU VAL PHE GLY LEU PRO LEU GLU THR VAL THR SER GLU GLN ARG ARG SER ALA LYS ALA ILE ASN PHE GLY LEU ILE TYR GLY MET SER ALA PHE GLY LEU ALA ARG GLN LEU ASN ILE PRO ARG LYS GLU ALA GLN LYS TYR MET ASP LEU TYR PHE GLU ARG TYR PRO GLY VAL LEU GLU TYR MET GLU ARG THR ARG ALA GLN ALA LYS GLU GLN GLY TYR VAL GLU THR LEU ASP GLY ARG ARG LEU TYR LEU PRO ASP ILE LYS SER SER ASN GLY ALA ARG ARG ALA ALA ALA GLU ARG ALA ALA ILE ASN ALA PRO MET GLN GLY THR ALA ALA ASP ILE ILE LYS ARG ALA MET ILE ALA VAL ASP ALA TRP LEU GLN ALA GLU GLN PRO ARG VAL ARG MET ILE MET GLN VAL HIS ASP GLU LEU VAL PHE GLU VAL HIS LYS ASP ASP VAL ASP ALA VAL ALA LYS GLN ILE HIS GLN LEU MET GLU ASN CYS THR ARG LEU ASP VAL PRO LEU LEU VAL GLU VAL GLY SER GLY GLU ASN TRP ASP GLN ALA HIS

## Citation

M. Teplova, S.T. Wallace, V. Tereshko, G. Minasov, A.M. Simons, P.D. Cook, M. Manoharan, M. Egli
Structural origins of the exonuclease resistance of a zwitterionic RNA
Proc. Nat. Acad. Sci. USA, 96, pp. 14240-14245, 1999.

## Space group

P 43

## Cell constants

         A =  102.650     B =  102.650     C =  86.300 (Ångstroms)
     alpha =  90.00   beta =  90.00  gamma =  90.00  (degrees)

## Crystallization conditions

- Method: vapor diffusion, sitting drop
- Drop: sodium citrate

## Refinement

The structure was refined using the CNS program. The R value is 22.4 for 16291 reflections in the resolution range 20.0 to 3.0 Å with Fobs > 2.0 sigma(Fobs).

## Coordinates

The coordinates for the asymmetric unit of this structure are stored in the NDB archive.

## Views of PD0080

A view of PD0080

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